diff --git a/tox.ini b/tox.ini
index 9b02d89ee0..8e29963bc8 100644
--- a/tox.ini
+++ b/tox.ini
@@ -1,7 +1,7 @@
 [tox]
 minversion = 3.1.0
 skipsdist = True
-envlist = validate,py27,py37,pep8,pip-install
+envlist = validate,py37,pep8,pip-install
 ignore_basepython_conflict=true
 
 [testenv]
@@ -13,11 +13,6 @@ deps = -c{toxinidir}/upper-constraints.txt
 commands =
   stestr run {posargs}
 
-[testenv:py27-check-uc]
-basepython = python2.7
-deps = -r{toxinidir}/upper-constraints.txt
-commands = check-conflicts {toxinidir}/upper-constraints.txt {toxinidir}/upper-constraints-xfails.txt
-
 [testenv:py36-check-uc]
 basepython = python3.6
 deps = -r{toxinidir}/upper-constraints.txt